Application of microsublimation for sample purification in compound-specific radiocarbon analysis

作者：Christian Heusser, Caroline Welte, Lukas Wacker, Kai Nakajima, Thomas M. Blattmann, Negar Haghipour, Timothy I. Eglinton · 发表于：Progress in Earth and Planetary Science · 年份：2025 · DOI：10.1186/s40645-025-00770-y · 被引用次数：2 · 研究领域：Archaeology and ancient environmental studies、Analytical Chemistry and Chromatography、Mass Spectrometry Techniques and Applications

Abstract This article presents the development and application of a microsublimation apparatus aimed at improving the purity of ultra-small samples for compound-specific radiocarbon analysis. Accurate radiocarbon ( 14 C) measurements require the effective isolation of biomarkers, yet procedural steps, such as chromatography and sample transfer, introduce contamination risks that can skew results. Here, we present a novel approach to remove contamination resulting from chromatographic isolation. The apparatus, constructed primarily from aluminum, allows solvent-free sublimation of multiple samples under vacuum. A constant contamination assessment showed a blank mass of 1.35 µg of carbon with a F 14 C of 0.33, indicating minimal contamination with 14 C-depleted carbon. The apparatus demonstrated high efficacy for compounds with higher melting points, such as amino acids and dyes, while compounds like alkanes showed lower recovery rates. These findings confirm the potential of microsublimation to enhance post-chromatography sample purity and improve the accuracy of 14 C measurements, though challenges remain for certain compound classes.