Taurochenodeoxycholic acid promotes abdominal fat deposition by modulating the crosstalk between bile acid metabolism and intestinal microbiota in broilers

作者：Xi Sun, Chaohui Wang, Xiaoying Liu, Yun Li, Zhouzheng Ren, Xiaojun Yang, Yanli Liu · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2025 · DOI：10.1186/s40104-025-01280-z · 被引用次数：5 · 研究领域：Animal Nutrition and Physiology、Rabbits: Nutrition, Reproduction, Health、Gut microbiota and health

BACKGROUND: The role of bile acids in modulating the gut microbiota and their impact on host metabolism has garnered significant attention. Taurochenodeoxycholic acid (TCDCA) is the predominant bile acid within the chicken bile acid pool and is closely related to metabolic disorders. The current study aims to investigate the potential effects of TCDCA on abdominal fat deposition in broilers. From 14 to 28 days of age, the broilers in the CON group received an oral administration of 1 mL of saline, while those in the treatment groups were administered 1 mL of a solution containing 0.05 g, 0.10 g, or 0.20 g of TCDCA. RESULTS: The results showed that TCDCA treatments from 14 to 28 d had no significant effects on BW, ADFI, ADG and FCR in broilers at the age of 28 days of age. However, the abdominal fat percentage in the 0.20 g TCDCA group significantly increased, accompanied by higher TBA and HDL-c levels, as well as a reduction in apolipoprotein B levels in serum. In addition, serum triglyceride levels tended to be higher in the 0.20 g TCDCA group (P = 0.098). The 0.20 g TCDCA treatment increased the gene expressions of SREBP-1, C/EBP-α, and ELOVL6, while decreasing the mRNA abundance of ATGL and CPT-1 in the abdominal fat. Serum levels of TCDCA, TDCA, and THDCA were significantly higher after 0.20 g TCDCA administration, while TCA levels were significantly lower, as determined by the targeted bile acid metabolomics analysis. Conversely, hepatic mRNA levels of CYP7A1, CYP27A1, B...