Advances in Serum-Free Suspension Culture Technology for Animal Cells and Their Applications

作者：Wei Ji, Ziyi Chen, Jinyu Zhou, Xinyu Yue, Zilin Qiao, Jiamin Wang · 发表于：Vaccines · 年份：2025 · DOI：10.3390/vaccines13111109 · 被引用次数：5 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects、Transgenic Plants and Applications、Protein purification and stability

Serum-free suspension culture technology for animal cells involves the division and proliferation of cells in serum-free medium as single cells or cell clusters within shaking flasks or bioreactors. This approach enables large-scale cell culture, enhances the yield and quality of biopharmaceuticals, reduces costs, and broadens the applications of animal cells. Serum-free suspension culture of adherent cells (e.g., Madin-Darby canine kidney (MDCK), Chinese hamster ovary (CHO), Vero, baby hamster kidney (BHK-21), and human embryonic kidney (HEK293) cells) has been successfully achieved through direct or indirect adaptation, medium optimization, and genetic engineering. Additionally, novel suspension cell lines, such as duck embryonic stem (EB66) and human retinoblastoma (PER.C6) cells, have been developed as potential new substrates for biopharmaceutical production. This review examines animal cell suspension culture technology and its applications in viral vaccines, recombinant proteins, and monoclonal antibodies, providing insights into the development and utilization of this important technology.