Bifunctional Fluorescent Probe for Simultaneous Imaging of H 2 S and Hcy Crosstalk and Therapeutic Evaluation in Acute Kidney Injury

作者：Ting Yu, Zhengze Long, Yang Li, Yu Wu, Xin Jiang, H. -Y. Jin, Han Tang, Danqing Xu, Youyu Zhang, Haitao Li, Peng Yin, Shouzhuo Yao · 发表于：Analytical Chemistry · 年份：2025 · DOI：10.1021/acs.analchem.5c05219 · 被引用次数：4 · 研究领域：Sulfur Compounds in Biology、Nanoplatforms for cancer theranostics、Molecular Sensors and Ion Detection

Acute kidney injury (AKI) is a critical clinical condition characterized by the rapid loss of renal function, requiring timely detection and intervention. Hydrogen sulfide (H 2 S) and homocysteine (Hcy) have been identified as key biomarkers in AKI, yet their dynamic interplay and coordinated regulation remain elusive. Herein, we report a bifunctional fluorescent probe ( Probe 1 ), based on coumarin derivatives, capable of real-time, simultaneous imaging of H 2 S and Hcy in distinct fluorescence channels with high sensitivity, selectivity, and rapid response. Probe 1 enables the concurrent detection of H 2 S and Hcy and was successfully applied to visualize both endogenous and exogenous levels in living cells. Notably, this probe achieved the first dual-channel imaging of H 2 S and Hcy fluctuations in cisplatin-induced AKI models, both in vitro and in vivo. Furthermore, it was employed to evaluate therapeutic responses to AKI-related drug treatments, demonstrating its value for dynamic monitoring of disease progression and intervention efficacy. This study presents the first chemical tool for investigating the synergistic roles of H 2 S and Hcy in AKI, offering new insights into sulfur-based signaling pathways and providing a promising platform for early diagnosis and therapeutic evaluation in AKI.