Comparative transcriptomic profiling of heat-tolerant and heat-sensitive chrysanthemum morifolium cultivars in response to heat stress

作者：Ziyan Song, Jutang Jiang, Yixue Zhang, Peng Chen, Kaiming Zhang, Fei Li, Yonghua Li‐Beisson · 发表于：Scientia Horticulturae · 年份：2025 · DOI：10.1016/j.scienta.2025.114305 · 被引用次数：3 · 研究领域：Plant Stress Responses and Tolerance、Light effects on plants、Photosynthetic Processes and Mechanisms

• The heat-tolerant cultivar ‘Weinisi’ exhibits a stronger antioxidant defense, with higher SOD/POD activity and lower MDA levels, reducing oxidative damage under heat stress. • Key HSP genes (HSP20, HSP40, HSP70) are significantly upregulated in ‘Weinisi’, enhancing its thermotolerance compared to ‘Kaisahong’. • Superior photosynthetic stability and metabolic adaptation in ‘Weinisi’ maintain pigment levels and soluble proteins, supporting resilience under high temperatures. Heat stress limits chrysanthemum growth and adversely affects both yield and cut-flower quality. However, the molecular mechanisms underlying heat tolerance in cut chrysanthemums remain unclear. In this study, we analyzed and compared physiological parameters, photosynthetic pigment and transcriptomic profiles of the heat-tolerant cultivar ‘Weinisi’ and the heat-sensitive cultivar ‘Kaisahong’ following heat treatment. The results showed that under heat stress, both chrysanthemum cultivars exhibited increased malondialdehyde (MDA) levels, with significantly higher MDA accumulation observed in ‘Kaisahong’ compared to ‘Weinisi’. Additionally, ‘Weinisi’ displayed higher activities of antioxidant enzymes such as SOD and POD, as well as elevated levels of soluble proteins and photosynthetic pigments, relative to ‘Kaisahong’. Transcriptome analysis revealed substantial differences in gene expression dynamics between the ‘Weinisi’ and ‘Kaisahong’ under high-temperature stress, with ‘Kaisahong’ exhibiting a more p...