Enhancing cross-organelle coordination to advance plant cytochrome P450 functionality in yeast

作者：Shanhui Xu, Songbo Wei, Yun Xiong, Xiaoxuan Teng, Kyle Sipe, Alair Abad, Théo Poucet, Yinsheng Wang, Yanran Li · 发表于：Science Advances · 年份：2025 · DOI：10.1126/sciadv.ady7184 · 被引用次数：5 · 研究领域：Plant biochemistry and biosynthesis、Photosynthetic Processes and Mechanisms、Microbial Metabolic Engineering and Bioproduction

Sustainable production of plant secondary metabolites remains a substantial challenge. Microbial cell factories offer a promising solution, yet reconstituting plant cytochrome P450s, key enzymes in these pathways, remains a major hurdle. In this study, we investigated a plant membrane protein, AtMSBP1, for its ability to broadly enhance P450 functionality in engineered yeast. Unlike in plants, interactions with cytochrome P450s are not essential for AtMSBP1's function in yeast. Specifically, AtMSBP1 orchestrates interorganelle communication, involving not only the endoplasmic reticulum but also mitochondria and vacuoles, supporting cytochrome P450s functionality in yeast. This study highlights the critical role of cross-organelle coordination in supporting cytochrome P450s functionality, emphasizing a shift from traditional ER-based approaches to engineering cross-organelle interactions and tailoring the cellular microenvironment to enhance plant cytochrome P450s functionality in yeast.