Colonization by Porphyromonas gingivalis in cervical squamous cell carcinomas promotes metastasis through FimA/CD151/ITGB1 signaling

作者：Xiuting Huang, Yuan Zhuang, Rui Wang, Jing Qu, Yuanyang Tan, Liang Li, Wenjun Pan, Huilong Nie, Kefeng Li, Yujing Lin, Ling Yi, Xiaoyu Feng, Huimin Lan, Guangxue Wang, Xian‐Tao Zeng, Zhenqi Jiang, De Yun Wang, Yongkang Qiao, Yan Yan · 发表于：Journal of Translational Medicine · 年份：2025 · DOI：10.1186/s12967-025-06928-y · 被引用次数：2 · 研究领域：Oral microbiology and periodontitis research、Gut microbiota and health、Cancer Research and Treatments

BACKGROUND: Cervicovaginal microbiota dysbiosis has been implicated in the progression of cervical cancer. Recent studies have reported an increased abundance of Porphyromonas species in the vaginal microbiota of cervical cancer patients. Porphyromonas gingivalis (P. gingivalis), a key periodontal pathogen, has been associated with adverse pregnancy outcomes and bacterial vaginosis; however, its potential role in the progression of cervical squamous cell carcinoma (CSCC) remains largely unexplored. METHODS: We employed immunohistochemistry (IHC), 16S rRNA fluorescence in situ hybridization (FISH), and immunofluorescence to detect P. gingivalis in CSCC tissues. The association between P. gingivalis colonization and CSCC patient survival outcomes was assessed. Transwell and wound healing assays were used to assess the migration and invasion abilities of CSCC cells. In vivo xenograft mouse models were established to evaluate the impact of P. gingivalis on tumor growth and metastasis. Pull-down assays were employed to investigate interactions between the P. gingivalis fimbrial protein FimA and host cell membrane proteins. RNA sequencing and Western blotting were utilized to identify signaling pathways activated by P. gingivalis in host cells. RESULTS: Porphyromonas gingivalis was detected immunohistochemically in 63% of CSCC tumor tissues, with significantly higher colonization in tumors compared to adjacent nontumor tissues (P < 0.0001). The presence of P. gingivalis was signifi...