Helicobacter pylori VacA modulates TRAF1-mediated 4-1BB/NF-kappaB axis to induce host apoptosis and chronic inflammatory damage

作者：Lingzhi Yuan, Shuoyi Yao, Nanfang Qu, Xinmeng Li, Xueer Yang, Aojian Deng, Minglin Zhang, Ting Cai, Lunxi Liang, Zhitao Liu, Xiaoming Liu, Fen Wang · 发表于：Molecular Medicine · 年份：2025 · DOI：10.1186/s10020-025-01349-5 · 被引用次数：7 · 研究领域：Helicobacter pylori-related gastroenterology studies、NF-κB Signaling Pathways、Genomics, phytochemicals, and oxidative stress

BACKGROUND: Helicobacter pylori (Hp) infection is the primary cause of gastric cancer. We previously demonstrated that TRAF1 expression was significantly increased during the multistep pathological timeline of Correa’s cascade and that these changes were closely correlated with Hp virulence factor VacA. However, the underlying mechanism remains unknown. METHODS: TRAF1-deficient or overexpressing human gastric mucosal epithelial cells were generated, and wild-type/vacA-KO mutant Hp, recombinant VacA protein and pDsRED2-N1-HA/VacA plasmid were used for mechanistic investigation of VacA. In addition, we established mice infection models and evaluated gastric pathological changes. RESULTS: RNA sequencing and cellular experiments suggested that positive correlations between TRAF1 expression and NF-κB pathway activation and apoptosis. VacA increased the protein levels of TRAF1, 4-1BB, p-IKKα/β, phosphorylated p65, Bax, and the downstream inflammatory factor IL-8 but reduced that of Bcl-xl, promoting apoptosis and inhibiting proliferation in vitro. Transient and stable overexpression of TRAF1 promoted VacA-induced 4-1BB expression, NF-κB pathways activation, apoptosis-related molecules levels, and downstream inflammatory factors secretion in gastric epithelial cells, whereas silencing of TRAF1 led to the opposite effects. Functionally, TRAF1 increased VacA-induced p65 nuclear translocation and transcriptional activity, leading to apoptosis. BAY11-7082 (an NF-κB inhibitor) or 4-1BB b...