Postbiotic supplementation promotes gut barrier integrity and immune balance in cats via microbiota modulation

作者：Weiwei Wang, Chengyi Miao, Qianqian Chen, Longjiao Wang, Xiaohan Chang, Lili Zhao, Wenqiang Ma, Shanyu Lei, Mengyao Ma, Yuqiang Zhang, Shuxing Chen, Lishui Chen, Ran Wang, Shaoyang Ge, Wei Xiong · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1692845 · 被引用次数：8 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Probiotics and Fermented Foods

Introduction Gastrointestinal disorders are common in cats and are closely linked to the gut microbiota, which supports immune function, metabolic balance, and barrier integrity. Although probiotics are beneficial, they can be unstable in pet foods. Postbiotics offer a safer and more stable alternative. Methods A postbiotic derived from Bifidobacterium animalis subsp. lactis was prepared by heat inactivation (95°C, 30 min), followed by centrifugation and freeze-drying. Eighteen healthy domestic cats were randomly assigned to a control group, a low-dose postbiotic group, or a high-dose postbiotic group for 28 days. Assessments included physical condition, immune status, gut barrier function, microbiota composition, and fecal short-chain fatty acids (SCFAs). Results Postbiotic supplementation improved fur condition and enhanced gut barrier integrity, as indicated by reduced levels of permeability markers such as diamine oxidase (DAO) and lipopolysaccharide (LPS). It also promoted immune function by increasing serum immunoglobulin G and anti-inflammatory cytokines IL-10 and IL-4. In addition, the intervention enriched beneficial bacteria such as Bifidobacterium spp. and elevated fecal concentrations of key SCFAs, including acetic, propionic, and butyric acids. Discussion These findings support the use of postbiotics as a safe and effective nutritional approach to help maintain gastrointestinal and immune health in cats.