Systemic proteomic rearrangements under combined hypoxia and arsenic stress drive metabolic shift towards re-aeration survival

作者：Vijay Kumar, Lara Vogelsang, Paulina Heinkow, Iris Finkemeier, Shanti S. Sharma, Karl‐Josef Dietz · 发表于：Journal of Experimental Botany · 年份：2025 · DOI：10.1093/jxb/eraf467 · 被引用次数：1 · 研究领域：Plant responses to water stress、Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms

Co-occurrence of hypoxia (Hpx) and arsenic (As) exposure is common in nature. Individual Hpx and As responses in plants have been scrutinized; however, only recent studies have revealed unique features of the combination (HpxAs). HpxAs exposure inflates cellular S demand to offset the highly oxidized cytosolic glutathione redox potential along with required As(III) sequestration. Further, a dual phosphate starvation response is recognized in HpxAs. The present study focused on the systemic influence of these HpxAs responses. HpxAs-exposed plants showed pronounced reduction in carbon assimilation (50%) and ferredoxin oxidation (40%), but accumulation of H2O2, Cys, and ascorbate (1.41-, 2.88-, and 2.88-fold, respectively) in leaves. A comprehensive proteomic and metabolomic analysis revealed a Hpx predominance in these systemic effects. Critically, despite lowered protein synthesis (rRNA levels), HpxAs plants showed a selective increase in 935 proteins (322 specific to HpxAs). Their in-depth analysis, in addition to accumulated abscisic acid (ABA) (4.37-fold) and cis-12-oxo-phytodienoic acid (OPDA) (22.2-fold) levels, helped identify S and P homeostasis-dependent alterations as primary drivers of systemic acclimatization, and the role of reactive oxygen species, ABA, and cis-OPDA/oxylipins in systemic signalling. The associated modulation of primary metabolism appears to aid HpxAs plants to endure the imminent re-aeration, thus inducing early flowering and completion of the lif...