Reovirus infection results in rice rhizosphere microbial community reassembly through metabolite-mediated recruitment and exclusion

作者：Zhanbiao Li, Wandi Luo, H. Xie, Cuiping Mo, Bixia Qin, Yige Zhao, Xiao Chen, Songbai Zhang, Yaling Zhao, Mengcen Wang, Yu Yang, Jianhe Cai, Baozhan Wang, Xu Liu, Yu Shi · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-025-02188-6 · 被引用次数：7 · 研究领域：Microbial Community Ecology and Physiology、Bacteriophages and microbial interactions、Plant-Microbe Interactions and Immunity

BACKGROUND: Microbial assembly plays a critical role in ecosystem function and biodiversity. While numerous studies have explored the effect of abiotic factors on the belowground community assembly, much less is known about the role of biotic interactions, particularly viral infections, in shaping microbial communities. Southern rice black-streaked dwarf virus (SRBSDV), a member of the Fijivirus genus in the Reoviridae family, has caused severe yield losses in rice due to its rapid transmission. However, its specific effects on rhizosphere microbiota and the dynamics of microbial community changes have not been fully elucidated. RESULTS: By leveraging metabolomics with amplicon and metagenomics, this study provided a comprehensive understanding of the effect of SRBSDV infection on the rhizosphere microbial community and their functions. The results revealed that SRBSDV invasion led to significant changes in rhizosphere metabolites and microbial assembly processes. Specifically, the estimated overdispersion of cations sharply decreased following viral infection, while anion levels decreased markedly during early infection and then increased rapidly after 15 days. Key taxa, such as methanotrophs (e.g., Methylomicrobium), nitrifiers (e.g., Nitrospira), and iron-cycling bacteria (e.g., Sideroxydans), not only increased in abundance but also showed strong involvement in the microbial assembly processes. These key microbes were closely linked to specific metabolites and organized i...