Research techniques of miRNA and its regulatory network under biotic and abiotic stresses in plants

作者：Yue Pan, Yuhao Peng, Yuhan Lu, Yuxia Yao, Wenjin Yu, Changxia Li · 发表于：Plant Stress · 年份：2025 · DOI：10.1016/j.stress.2025.101103 · 被引用次数：4 · 研究领域：Plant Molecular Biology Research、Plant Genetic and Mutation Studies、Plant Stress Responses and Tolerance

• The expression patterns of microRNAs (miRNAs) under stress conditions are complex. • There are several key technologies for exploring the mechanism of action of miRNA. • miRNA enhances the resistance of plants by regulating multiple signaling pathways. Biological and abiotic stresses exert adverse effects on plant productivity. Consequently, elucidating the molecular mechanisms underlying responses to these stresses is essential. The expression patterns of microRNAs (miRNAs) under biological and abiotic stress conditions exhibit significant diversity and complexity. However, the mechanism by which miRNAs participate in plant stress resistance responses and regulate associated target genes requires more comprehensive and systematic elucidation. This review systematically states biosynthesis mechanisms of miRNAs, we describe Pol II-mediated primary miRNA transcript generation and DCL1 complex-mediated stepwise processing. At the technical level, classic detection methods such as Northern blotting and Real-time qPCR are detailed, while omics techniques including next-generation bioinformatics platforms and degradome analysis are integrated. Moreover, review systematically introduces the dynamic regulatory networks of plant miRNAs under biotic and abiotic stress. In terms of miRNA stress response mechanisms, novel insights have emerged regarding the enhancement of plant stress resistance through regulation of key antioxidant enzymes (SOD, POD), activation of plant hormone signa...