Genome-assisted identification of wheat leaf rust resistance gene Lr.ace-4A/Lr30

作者：Jinwei Yang, Hongna Li, Mengyu Li, Rui Song, Tao Shen, Guiping Wang, Dong Xu, Ming Hao, Aolin Jia, Shamsur Rehman, Lei Hua, Yanyan Liang, Cheng Chi, Caixia Lan, Xing Wang Deng, Jorge Dubcovsky, Baoxing Song, Xiaodong Wang, Shisheng Chen · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-64428-5 · 被引用次数：8 · 研究领域：Wheat and Barley Genetics and Pathology、Plant Disease Resistance and Genetics、Yeasts and Rust Fungi Studies

Leaf rust is a devastating disease of wheat. Growing rust-resistant wheat varieties is the best strategy to mitigate this threat. Here, we generate a 10.51-gigabase chromosome-scale assembly of the durum wheat landrace PI 192051. Using mutagenesis and transcriptome sequencing, we identify the leaf rust resistance gene Lr.ace-4A within a recombination-sparse region of PI 192051 and demonstrate that Lr.ace-4A is identical to the previously designated Lr30 gene in hexaploid wheat. Lr.ace-4A/Lr30 encodes a non-canonical coiled-coil nucleotide-binding leucine-rich repeat receptor, featuring tandem nucleotide-binding domains. This gene is both necessary and sufficient to confer resistance to leaf rust, as demonstrated by CRISPR/Cas9-induced mutations and transgenic complementation. Lr.ace-4A provides near-immunity resistance in durum wheat, though its effectiveness is diminished in hexaploid wheat. Two amino acid polymorphisms differentiate the resistant and susceptible Lr.ace-4A haplotypes, with transgenic plants carrying either susceptible variant showing susceptibility. The cloning of Lr.ace-4A will accelerate its deployment in wheat breeding programs.