ATOX1 overexpression mitigates copper homeostasis in microglia: Implications for Alzheimer's disease therapy

作者：Fuxin Zhong, Jiani Wu, Zhangjing Deng, Wuhan Yu, Jiaqi Song, Yingxi Chen, Weihua Yu, Yang Lü · 发表于：Genes & Diseases · 年份：2025 · DOI：10.1016/j.gendis.2025.101888 · 被引用次数：3 · 研究领域：Alzheimer's disease research and treatments、Trace Elements in Health、Nuclear Receptors and Signaling

Copper (Cu 2+ ) is a known contributor to the pathogenesis of Alzheimer's disease (AD). However, it is uncertain whether proteins regulating copper homeostasis affect Cu 2+ in microglia. Antioxidant protein 1 (ATOX1) plays a key role in Cu 2+ homeostasis, oxidative stress, and cell protection. Despite its critical functions, the role of ATOX1 in AD pathology remains poorly defined. This study aims to examine the effects of ATOX1 on oxidative stress, apoptosis, and neuroinflammation in microglia by modulating Cu 2+ homeostasis. In vivo , a 5×FAD mouse model was used to investigate the localization and expression of ATOX1 in AD by immunofluorescence and three-dimensional reconstruction. The Aβ 1-42 oligomer was used to establish an AD model in vitro . The role of ATOX1 in Cu 2+ homeostasis regulation in microglia was further studied using co-immunoprecipitation, Western blotting, quantitative real-time PCR, and spectrophotometry. A reduction in ATOX1 expression was noted in Aβ-plaques-associated microglia compared with normal microglia. Cu 2+ levels were elevated in the in vitro AD model, and ATOX1 directly regulated copper homeostasis via P1B-ATPase (ATP7B) in microglia. Excessive Cu 2+ induced oxidative stress, neuroinflammation, and apoptosis. Overexpression of ATOX1 alleviated this neurotoxicity, indicating its potential to alleviate oxidative stress, cell apoptosis, and neuroinflammation in AD. ATOX1 overexpression offers protective effects on microglia through Cu 2+ homeo...