RNA-Seq and Single-Cell RNA-Seq Analyses of Tilapia Head Kidney in Response to Streptococcus agalactiae and Aeromonas hydrophila

作者：Qi Li, Zulin Fang, Zhongyu Li, Xinxian Wei, Youchuan Wei · 发表于：Animals · 年份：2025 · DOI：10.3390/ani15202951 · 被引用次数：4 · 研究领域：Aquaculture disease management and microbiota、Ichthyology and Marine Biology、Fish biology, ecology, and behavior

High-throughput sequencing has significantly advanced the exploration of fish immune mechanisms, enabling a more detailed understanding of immune responses and their underlying molecular pathways. In this study, we applied comparative transcriptomics and single-cell RNA sequencing to investigate the immune mechanisms of tilapia in response to different pathogenic bacteria. Our results demonstrated that nonspecific cytotoxic cells (NCCs) and monocytes/macrophages (Mos/Mφs) mounted the most pronounced responses to both Streptococcus agalactiae and Aeromonas hydrophila infections. Moreover, Mos/Mφs exhibited distinct differentiation patterns depending on the bacterial challenge. Collectively, these findings offer new insights into the antibacterial immune strategies of lower vertebrates.