Mechanisms of pyrethroid resistance in Culicidae mosquitoes from Hainan Island, China

作者：Qingyun Huang, Yunfei Zhou, Jiabao Xu, Guofa Zhou, Qiaolu Guo, Yixuan Duan, Guiyu Zheng, Menglou Zhu, Saifeng Zhong, Daibin Zhong, Faxing Fu, Wen Si, Yu Jiang, Tianya He, Suhua Liu, Gang Lu, Yiji Li, Tingting Li · 发表于：Parasites & Vectors · 年份：2025 · DOI：10.1186/s13071-025-07052-y · 被引用次数：2 · 研究领域：Malaria Research and Control、Mosquito-borne diseases and control、Insect Pest Control Strategies

BACKGROUND: Mosquito-borne diseases represent critical global public health threats. Insecticide-based prevention and interventions remain essential for disease and vector management. However, insecticide resistance in mosquitoes threatens the effectiveness of these management measures. This study investigated the susceptibility to pyrethroid insecticides and associated resistance mechanisms in five dominant mosquito populations on Hainan Island, China. METHODS: World Health Organization (WHO) tube bioassays were conducted to evaluate insecticide resistance profiles in Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus, Aedes aegypti, and Culex tritaeniorhynchus. We assessed the synergistic effects of pre-exposure to 4% piperonyl butoxide (PBO) on deltamethrin mortality rates in Aedes albopictus and Culex quinquefasciatus populations. We genotyped kdr alleles at codon 1534 of the voltage-gated sodium channel gene in Ae. albopictus and at codon 1014 in Cx. quinquefasciatus. RESULTS: All five mosquito species exhibited significant resistance to pyrethroid insecticides. Ae. albopictus populations from seven localities were resistant to 0.25% permethrin, 0.03% deltamethrin, and 0.03% alpha-cypermethrin, with mortality rates ranging from 0% to 35.0%, 4.0% to 51.0%, and 2.0% to 27.0%, respectively. Similarly, Cx. quinquefasciatus populations from five sites demonstrated resistance to 0.25% permethrin, 0.4% deltamethrin, and 0.5% alpha-cypermethrin, with all mortality ra...