Generation of a pseudo-timeline describing progressive human exocrine and endocrine pancreatic pathology in cystic fibrosis through novel semi-quantitative scoring and AI-driven quantitative image analysis

作者：Yara Al-Selwi, Dina Tiniakos, Sarah J. Richardson, Christine S. Flaxman, Lydia Russell, Rowan Coulthard, Rashmi R Maheshwari, Nicola Dyson, Minna Honkanen‐Scott, Günter Klöppel, James Shaw, Nicole Kattner · 发表于：Diabetologia · 年份：2025 · DOI：10.1007/s00125-025-06559-4 · 被引用次数：4 · 研究领域：Cystic Fibrosis Research Advances、Pancreatitis Pathology and Treatment、Pancreatic and Hepatic Oncology Research

AIMS/HYPOTHESIS: Cystic fibrosis (CF) is associated with pancreatic exocrine insufficiency (PEI) early in life and diabetes in at least 50% of adults. Underlying CF-related changes within the pancreas remain incompletely understood due to scarcity of available human tissue, protracted disease course and absence of robust and reproducible analytical approaches. This study aimed to develop and apply a systematic analysis cross-sectionally to CF pancreatic tissue samples to construct a timeline of exocrine and endocrine changes with progressive disease. METHODS: Through a pathologist-led iterative approach, a light-microscopy semi-quantitative scoring system and artificial-intelligence-driven quantitative image analysis for individual pancreatic variables were developed. These were applied to human pancreatic tissue from 29 CF and 58 control donors without pancreatic disease. RESULTS: Rapid loss of acinar tissue with virtually complete absence and replacement by adipocytes by the age of 7 years was confirmed. Ductal blockage by thickened secretions was associated with increasing ductal dilatation accompanied by periductal fibrosis, followed by ductal loss with involution of associated fibrosis in parallel with increasing adipocyte proportional area. Remaining ducts were relatively small, surrounded by residual fibrosis. Islets became increasingly clustered, initially surrounded by pancreatic stellate cells and fibrosis, then disorganised by interposing fibrotic tissue between en...