Gut microbiota and metabolite changes in metastatic colorectal cancer via 16S rRNA and metabolomics

作者：Deng Pan, Lin Hua, Zeng Hu, Feng Guo · 发表于：World Journal of Gastrointestinal Oncology · 年份：2025 · DOI：10.4251/wjgo.v17.i10.111509 · 被引用次数：3 · 研究领域：Metabolomics and Mass Spectrometry Studies、Gut microbiota and health、Immune cells in cancer

BACKGROUND Distant metastasis causes most colorectal cancer (CRC) deaths. Gut microbiota (GM) dysbiosis and altered metabolites drive metastasis progression, serving as potential diagnostic biomarkers. AIM To investigate alterations in GM and metabolites between patients with non-metastatic and distant metastatic CRC. METHODS According to the inclusion criteria, fresh fecal samples were collected from 14 non-metastatic CRC patients and 15 distant metastatic CRC patients. We performed 16S rRNA sequencing to analyze the composition, diversity, and differential abundance of GM, along with predictive functional profiling of microbial communities. Additionally, all samples underwent liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomic sequencing to identify metabolic changes and predict their biological functions. RESULTS The cohort comprised 16 non-metastatic CRC patients (designated as the S group) and 16 distant metastatic CRC patients (designated as the DZ group). Sequence analysis (16S rRNA) identified a total of 35016 operational taxonomic units (OTUs) across both groups (16886 OTUs in the S group; 16270 in the DZ group; 1860 shared between groups). Inter-group microbial diversity analysis revealed notable differences in the β-diversity group (P < 0.05). Comparative analysis of GM revealed significant taxonomic composition differences between groups (P < 0.05), with higher relative abundances of Butyricicoccus , Ruminococcus_1 , Coprococcus_2 in...