Effects of Bioactive Glass Carrier-Loaded Sacubitril/Valsartan Sodium Tablets on Cardiomyocytes From Rats With Chronic Heart Failure

作者：Jia Fan, Yi Ma, Xuebin Geng, Li Li · 发表于：International Journal of Pharmacology · 年份：2025 · DOI：10.31083/ijp44207 · 研究领域：Tissue Engineering and Regenerative Medicine、Electrospun Nanofibers in Biomedical Applications、Cardiac Ischemia and Reperfusion

Background and Objective: Sacubitril/valsartan sodium tablets (SVSTs) represent a therapeutic option for chronic heart failure (CHF), functioning by inhibiting angiotensin II receptors and neprilysin. Thus, this study aimed to develop mesoporous nano-bioactive glass (MNBG)-loaded SVSTs to enhance the bioavailability of SVSTs and investigate the treatment effects on myocardial cell autophagy and apoptosis in rats with CHF. Materials and Methods: The MNBG-loaded SVSTs were prepared and tested in normal Sprague-Dawley rats (Ctrl group) and CHF models created through abdominal aortic ligation. The rats were divided into CHF, SVST, and SVST/MNBG groups (15 rats each). Cardiac function, myocardial tissue pathology, and the levels of reactive oxygen species (ROS), nitric oxide (NO), creatine kinase-MB (CK-MB), N-terminal pro-B-type natriuretic peptide (NT-proBNP), apoptosis-related proteins (Bcl-2 and Bax), and autophagy-related proteins (Atg5, beclin1, p62, and LC3II/I) were assessed. Statistical analysis was performed using SPSS 26.0 via one-way analysis of variance (ANOVA), t-test, and χ2 test (p < 0.05). Results: The CHF group demonstrated reduced cardiac function with increased ROS, CK-MB, NT-proBNP levels, and decreased NO levels, along with altered protein expression (decreased Bcl-2, Atg5, beclin1, p62, and increased Bax, LC3II/I). The SVST and SVST/MNBG groups presented improved cardiac function, reduced ROS, CK-MB, and NT-proBNP levels, increased NO, and favorable prote...