A DNA-containing phase-separated compartment links the nuclear envelope to chromosome axes to promote homolog pairing in C. elegans

作者：Ruoxi Wang, Yuqi Tian, Libo Liu, Jian-Hua Wang, Li Zhao, Yuanyuan Liu, Feifei Qi, Liangran Zhang, Jun Zhou, Meng‐Qiu Dong, Jinmin Gao · 发表于：Science Advances · 年份：2025 · DOI：10.1126/sciadv.adw5764 · 被引用次数：2 · 研究领域：DNA Repair Mechanisms、RNA Research and Splicing、Nuclear Structure and Function

, chromosome pairing centers (PCs) and PC-recruited proteins mediate homolog recognition and pairing. Here, we further dissect the molecular characteristics of this system and reveal the involvement of a phase-separated compartment in pairing. Focusing on the PC protein HIM-8, we explored its functional domains in vivo and its self-multimerizing properties in vitro. We find that the amino-terminal domain facilitates PC chromatin binding of the carboxyl-terminal domain, whereas the middle unstructured region is essential for PC association with the nuclear envelop (NE). Integrated analyses suggest that PC-NE association is mediated by a DNA-containing phase-separated compartment that is spatially distinct from, but connected to, the chromosome axis-likely through flexible linkages. We propose that homology evaluation occurs within this compartment, where direct DNA-DNA interactions could play a role.