Differential regulation of soil antibiotic resistance genes by biochar types and their derived dissolved organic matter

作者：Yi Chen, Weili Jia, Rui Ma, Yi‐Hao Yu, Lu-Kai Qiao, Fang-Zhou Gao, Guang‐Guo Ying · 发表于：Soil & Environmental Health · 年份：2025 · DOI：10.1016/j.seh.2025.100179 · 被引用次数：6 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Soil Carbon and Nitrogen Dynamics、Microbial Community Ecology and Physiology

Agricultural soil represent a critical hotspot for the emergence and dissemination of antibiotic resistance genes (ARGs), posing significant threats to food safety, public health, and agricultural sustainability. Biochar, a carbon rich material derived from biomass pyrolysis, has emerged as a promising soil amendment capable of modulating the fate and transport of ARGs in terrestrial ecosystems. Nevertheless, the underlying mechanisms by which biochar and its associated dissolved organic matter (biochar-derived dissolved organic matter, BDOM) influence ARG dynamics remain poorly understood. It is hypothesized that biochar and BDOM regulate ARG dissemination through their effects on soil microbial community composition, functional potential, and metabolic activity. To test this hypothesis, we conducted a controlled microcosm experiment in which corn stover biochar (CBC), reed straw biochar (RBC), corn stover BDOM (CBDOM), and reed straw BDOM (RBDOM) were applied to agricultural soil amended with organic fertilizer. Our results revealed contrasting effects of the two biochar types: CBC increased the relative abundance of ARGs by up to 2.48-fold ( p < 0.05) compared to organic fertilizer control, whereas RBC consistently suppressed ARG levels by up to 91%. In contrast, BDOM exhibited a comparatively weaker influence on ARG abundance than its solid-phase biochar counterpart. Partial least-squares path modeling identified mobile genetic elements as the primary drivers of ARG disse...