Distinct Suppression of Prednisone on Endometrial Immune Cells in Women With Reproductive Failure

作者：Lu Chen, Xiaoyan Chen, Qianhan Xu, Joshua Li, Wen Yang, Hanbin Wu, Chi Chiu Wang, Xiaodan Fan, Jie Ji, Jacqueline Pui Wah Chung, Ming‐Qing Li, Tin Chiu Li, Tao Zhang · 发表于：American Journal of Reproductive Immunology · 年份：2025 · DOI：10.1111/aji.70151 · 被引用次数：6 · 研究领域：Reproductive System and Pregnancy、Gynecological conditions and treatments、Endometriosis Research and Treatment

RESEARCH QUESTION: For women with reproductive failure, prednisone is widely used but remains controversial as a therapy for improving clinical outcomes. This study aimed to investigate the impact of prednisone on various endometrial immune cells. DESIGN: A total of 24 women with repeated implantation failure and recurrent miscarriage underwent their first endometrial biopsy precisely 7 days after luteinizing hormone surge (LH surge +7). Prednisone was administered from the first day of the subsequent menstrual cycle, followed by the second endometrial sampling on day LH surge +7 again. The density and cell-cell clustering of CD3-CD56+CD16- NK cells, CD3-CD56+CD16+ NK cells, CD68+CD16- macrophages, CD68+CD16+ macrophages, CD3+CD56- T cells, and CD3+CD56+ NK-T cells were analyzed by multiplex staining and compared before and after prednisone treatment. RESULTS: Following prednisone treatment, a significant decrease in the percentage of CD3-CD56+CD16- NK cells (p < 0.001), CD68+CD16- macrophages (p = 0.007), and their clustering degree (p = 0.038) was observed. No significant changes were noted in CD3-CD56+CD16+ NK cells, CD68+CD16+ macrophages, CD3+CD56- T cells, CD3+CD56+ NK-T cells, and their cell-cell clustering. CONCLUSION: Prednisone does not generally reduce all endometrial immune cells; instead, it selectively suppresses specific immune cell subtypes in women with reproductive failure.