Lipoxygenase ZmLOX3 Enhances Salt Tolerance of Maize Under the Regulation of ZmNAC032

作者：Xinyang Che, Yulei Wei, X. Wang, Xinqi Wang, Zixuan Wu, Jie Deng, Shengnan Ge, Xinyu Liu, Zhicheng Cai, Haiyang Zhang, Lin He, Jingyu Xu · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.5c02349 · 被引用次数：4 · 研究领域：Plant Stress Responses and Tolerance、Plant Disease Resistance and Genetics、Plant Pathogens and Resistance

Lipoxygenase (LOX) plays a critical role in plant biotic and abiotic stress responses by mediating lipid peroxidation and the production of jasmonic acid (JA). In this study, maize ZmLOX3 was identified as a positive regulator in salt stress tolerance. Overexpression of ZmLOX3 enhanced the salt tolerance of Arabidopsis . When maize seedlings were subjected to salt stress, the ZmLOX3 OE lines exhibited a better growth phenotype than the control (B104) and the zmlox3 CR (CRISPR/Cas) knockout mutants. Overexpression of ZmLOX3 improved ROS scavenging, Na + /K + homeostasis, and cell membrane stability. Transcriptome analyses revealed that ZmLOX3 OE triggered the expression of genes involved in both the JA synthesis and signaling pathways. A transcription factor ZmNAC032 was identified via Y1H screening and was able to bind to the C[A/G]CG[T/G] sequence in the ZmLOX3 promoter and activate its expression. These findings are helpful for deciphering the function and regulatory status of ZmLOX in improving salt tolerance.