Fatty acid synthase-mediated lipid droplet formation enhances macrophage killing of Staphylococcus aureus

作者：Yanping Wu, Yanping Wu, Jiaxin Shen, Shenwei Gao, Miao Li, Qingyu Weng, Kua Zheng, Chen Zhu, Zhongnan Qin, Jiafei Lou, Jiafei Lou, Songmin Ying, Yinfang Wu, Yinfang Wu, Zhihua Chen, Wen Li · 发表于：Cell Death and Disease · 年份：2025 · DOI：10.1038/s41419-025-08044-7 · 被引用次数：3 · 研究领域：Metabolomics and Mass Spectrometry Studies、Influenza Virus Research Studies、Lipid metabolism and biosynthesis

Macrophages play a critical role in defending against Staphylococcus aureus (S. aureus), a major human pathogen. Recently, there has been growing interest in the metabolic regulation of macrophage function; however, the specific role of lipid synthesis in macrophage activation remains poorly understood. This study demonstrates that fatty acid synthase (FASN), an enzyme integral to de novo lipogenesis, is significantly upregulated in macrophages during S. aureus infection. Notably, S. aureus engages in a functional interaction with proteasomes, inhibiting their activity through the PI3K/AKT/mTOR signaling pathway. This interaction results in reduced degradation of FASN, leading to elevated levels of this crucial enzyme. The increased expression of FASN is vital for macrophage-mediated pathogen clearance, as it facilitates the formation of lipid droplets (LDs), which in turn enhance the antimicrobial response against S. aureus, partly through the accumulation of the antimicrobial peptide CAMP. In a murine pneumonia model, deficiency of FASN correlates with increased bacterial burden, exacerbated lung inflammation, and a significant reduction in survival rates. Collectively, these findings underscore the essential role of FASN-mediated LD formation in macrophage activation and highlight potential therapeutic targets within the FASN and lipid metabolism pathways for the treatment of S. aureus pneumonia.