Auxin/ ROS ‐Mediated Coordination of Lateral Root and Root Hair Development in Wheat ( Triticum aestivum L.) Under Low‐Temperature Stress

作者：Wenyuan Shen, Xinyu Chen, Qingming Ren, Yunfei Wu, Xurun Yu, Fei Xiong · 发表于：Physiologia Plantarum · 年份：2025 · DOI：10.1111/ppl.70563 · 被引用次数：4 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant nutrient uptake and metabolism

Low temperature is a common stressor for winter field crops, notably impacting wheat roots. However, there is scarce knowledge on subterranean wheat root responses to low temperatures. To address this knowledge gap, we investigated the adaptive responses and underlying mechanisms of wheat roots to cold stress using a hydroponic system. Our investigation revealed that 4°C stress alters wheat root system architecture (RSA) by inhibiting primary and lateral root growth while increasing root hair density and length. Transcriptomic analysis identified auxin (IAA) and reactive oxygen species (ROS) as potentially crucial regulatory factors in the adaptation of wheat roots to low temperatures. Furthermore, a protein interaction network links ROS metabolism and auxin signaling, with NADPH-dependent thioredoxin reductase A (NTRA) serving as a central regulatory node. Pharmacological manipulation (utilizing IAA and polar transport inhibitors) and histochemical localization experiments demonstrated that the interplay between lateral roots and root hairs under low-temperature stress is associated with the spatial distribution of IAA in the epidermis and stele. Time-course analysis indicated that ROS function as early signaling molecules that initiate downstream IAA signaling pathways. Pharmacological intervention using ROS scavengers and histochemical localization experiments revealed that scavenging ROS disrupts the epidermal distribution of auxin, consequently impeding root hair develop...