Integrative genomics reveals PDE11A and SAAL are key regulators of growth in Pacific white shrimp (Penaeus vannamei)

作者：Lupeng Zhang, Hao Wang, Mingxuan Teng, Qianqian Zhao, Mingyang Zhao, Yongyu Huang, Wei-Hua Yan, Jilong Ouyang, Zhenmin Bao, Qifan Zeng · 发表于：Water Biology and Security · 年份：2025 · DOI：10.1016/j.watbs.2025.100474 · 被引用次数：2 · 研究领域：Aquaculture Nutrition and Growth、Invertebrate Immune Response Mechanisms、Protein Hydrolysis and Bioactive Peptides

Pacific white shrimp ( Penaeus vannamei ) is a globally important aquaculture species, prized for its adaptability, high yield, and economic value. Growth traits are key determinants of aquaculture profitability, making it essential to understand their genetic basis. In this study, we performed whole-genome sequencing of 996 individual P. vannamei , identifying 2,706,353 high-quality SNPs through genotyping and imputation. A genome-wide association study (GWAS) revealed six quantitative trait loci (QTLs) on linkage group (LG) 2, 25, 26, and 37, with 30 SNPs significantly associated with body length. The most significant associations were located on LG37, encompassing PDE11A , SAAL , IAO-like , and several other genes potentially involved in molting and growth regulation. Functional validation through RNA interference demonstrated that silencing PDE11A and SAAL significantly reduced growth, confirming their critical role in growth modulation. This integrative approach, combining a GWAS with functional genomics, sheds light on the molecular mechanisms governing in P. vannamei . The identification and validation of PDE11A and SAAL as key growth-related genes offer valuable insights for developing selective breeding strategies to improve growth traits in aquaculture species. • A genome-wide association study of 996 individual Penaeus vannamei identified 30 SNPs linked to body length. • PDE11A and SAAL were implicated as key growth regulators, validated through RNA interference ex...