Effects of beet pulp on growth performance, intestinal health and bile acid metabolism of largemouth bass (Micropterus salmoides)

作者：Like Zhang, Jiayin Zhang, Hang Zhou, Beiping Tan, Shuyan Chi, Shiwei Xie, Junming Deng · 发表于：Aquaculture Reports · 年份：2025 · DOI：10.1016/j.aqrep.2025.103136 · 被引用次数：2 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Protein Hydrolysis and Bioactive Peptides

A 56-day feeding trial was conducted to evaluate the effects of dietary beet pulp (BP) on growth performance, intestinal health, and bile acid metabolism in juvenile largemouth bass ( Micropterus salmoides ). Six isoproteic and isolipidic diets (designated as BP0, BP3, BP6, BP9, BP12, and BP15) were formulated to contain 0 %, 3 %, 6 %, 9 %, 12 %, and 15 % BP, respectively. Following random allocation, a total of six dietary treatments were established, each containing juvenile fish with a mean initial body weight of 10.72 ± 0.01 g. Although dietary BP inclusion at 3 %-6 % exerted no significant effect on growth, the 3 % inclusion level enhanced intestinal function by: (1) increasing the activities of amylase and superoxide dismutase; (2) up-regulating the transcription of genes encoding Occludin, Claudin-4, and ZO-1; and (3) modulating inflammatory responses through elevated IL-10 expression. Meanwhile, hepatic bile acid homeostasis was effectively regulated through the FXR-SHP signaling axis. In contrast, dietary BP levels exceeding 9 % significantly impaired growth performance, exacerbated intestinal oxidative damage, down-regulated Claudin-4 expression, and up-regulated TNF-α. Moreover, 15 % BP inclusion induced cholestasis, resulting in severe liver damage and functional impairment. Therefore, a 3 % dietary BP inclusion is recommended as optimal for largemouth bass. • This study investigates the effects of beet pulp in largemouth bass feeds. • Dietary inclusion of 3 % bee...