Clinical and laboratory profiles of Oropouche virus disease from the 2024 outbreak in Manaus, Brazilian Amazon

作者：Maria Paula Gomes Mourão, Gisely Cardoso de Melo, Joabi Nascimento, Victor Irungu Mwangi, Lívia Sacchetto, Luiz Gustavo Gardinassi, Rebeca Linhares Abreu Netto, J.K.M. Mota, Sérgio Damasceno Pinto, Mayara Mansur Fernandes Tavares, Liz Moreira Cordeiro, Barbara Seffair de Castro, de Abreu Brasil, Dyandra dos Santos Porto, Bianca Oliveira, Ana Carolina Shuan Laco, Lorenna Pereira de Souza, Karolaine Oliveira Bentes, Márcia Castilho, Carlos Eduardo de Morais, Heline Silva Lira, Luís Felipe Alho, Flor Ernestina Martínez-Espinosa, Vanderson de Souza Sampaio, Felipe Murta, Marco Aurélio Sartim, Maurício Lacerda Nogueira, Marcus Lacerda, Wuelton Marcelo Monteiro · 发表于：PLoS neglected tropical diseases · 年份：2025 · DOI：10.1371/journal.pntd.0013604 · 被引用次数：7 · 研究领域：Mosquito-borne diseases and control、Viral Infections and Vectors、Viral Infections and Outbreaks Research

BACKGROUND: The 2024 Oropouche virus (OROV) outbreak in Brazil raised public health concerns due to its unprecedented rapid spread, high incidence, and potential neurological complications. OROV symptoms overlap with locally endemic arbovirus diseases, like dengue virus (DENV), complicating diagnosis. The study aimed to compare clinical, laboratory, and immunological profiles in OROV and DENV cases, crucial for improving diagnosis and management. METHODS: This study analyzed 51 OROV and 78 of DENV cases consecutively enrolled in Manaus, Amazonas, Brazil, and monitored for 28 days. OROV diagnosis was performed by real-time PCR (RT-PCR) using serum and urine samples. OROV RT-PCR positive samples were genotyped. A paired Plaque Reduction Neutralization Test (PRNT) was conducted on samples collected at D1 and D28. Patients with a ≥ 4-fold increase in neutralizing antibody titer between D1 and D28 were considered OROV-positive. Clinical manifestations, hematology, biochemistry, and cytokine profiles were analyzed. Statistical analysis included comparison between OROV and DENV patients. RESULTS: Genome sequencing of OROV isolates confirmed presence of a previously reported novel reassortment event, consistent with ongoing localized transmission. Urine RT-PCR demonstrated low positivity compared to serum samples. The paired PRNT increased sensitivity in 45%. Clinically, OROV infection was associated with significantly higher frequencies of severe headache, myalgia, arthralgia, and r...