An activity-based probe library for identifying promiscuous amide hydrolases

作者：Chathuri J. Kombala, Jared O. Kroll, Lucas Webber, Stephen Callister, Natalie Sadler, Erin Bredeweg, Irlanda N Medrano, Kristoffer Brandvold, Sankarganesh Krishnamoorthy · 发表于：Chemical Communications · 年份：2025 · DOI：10.1039/d5cc04162g · 研究领域：Chemical Synthesis and Analysis、Analytical Chemistry and Chromatography、Computational Drug Discovery Methods

A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to putative hydrolases. This approach prioritizes candidate hydrolases for further experimental validation with potential applications in the environment, biomanufacturing, and medicine.