The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer

作者：Ting Ding, Yanjun Diao, Ruiqing Fu, Chengxiang Gong, Qiye He, Weixiang He, Longlong Zhang, Xiaojian Yang, Xianfei Zeng, Yu Lijuan, Jiayun Liu, Weimei Shi, Kang Zhang, Xiaoke Hao · 发表于：Journal of Advanced Research · 年份：2025 · DOI：10.1016/j.jare.2025.09.056 · 被引用次数：5 · 研究领域：Extracellular vesicles in disease、Prostate Cancer Treatment and Research、Prostate Cancer Diagnosis and Treatment

This study confirmed the significance of EV DNA methylation targets in prostate cancer (PCa) diagnosis and classification. We developed and optimized a DNA methylation detection system for extracellular vesicles (EVs), performed an extensive genome-wide screening of differential methylation targets, and created a PCa-specific targeted sequencing panel. Notably, we constructed, for the first time, a differential diagnostic model for PCa based on urine EV DNA methylation. This model demonstrated unique advantages in distinguishing aggressive PCa cases, thereby providing new insights into tumor markers and offering a promising avenue for enhancing prostate cancer diagnosis. • EV DNA accurately reflects the methylation state of source cell genomic DNA, providing a reliable substitute for tissue detection in PCa. • Total EV without DNase I treatment is a preferable choice for detecting EV DNA methylation biomarkers. • A novel panel of six methylation sites (MACF1、LINC01359-1、LINC01359-2、ADCY4、GAPLINC、C19orf25) in urine EV DNA demonstrates unique advantages in distinguishing PCa patients, particularly those with aggressive disease. As one of the most common malignant tumors in men, prostate cancer (PCa) still lacks convenient, non-invasive and highly specific diagnostic markers. The advantages of Extracellular vesicle (EV) DNA in tumor diagnosis have gradually attracted the attention of researchers. However, methylation detection, which is more advantageous than mutation detection ...