Effects of green light replacing some red and blue light on the healing and growth of two-stage-grafted tomato seedlings

作者：Guoxiu Wu, Yiran Li, Jiazi Zhang, Shuhua Liu, Xueying He, Dandan Cui, Yang Li, Yanman Li, Fan Wang, Shengli Li · 发表于：Scientia Horticulturae · 年份：2025 · DOI：10.1016/j.scienta.2025.114420 · 被引用次数：3 · 研究领域：Plant Disease Management Techniques、Light effects on plants、Plant tissue culture and regeneration

• This study examined healing issues during high-density healing and root regeneration during the cutting stage of two-stage grafting cultivation. • The addition of 20% and 30% green light to a red:blue light ratio of 7:3 (R7B3G2 and R7B3G3) during high-density healing resulted in the highest survival rates. • Green light improved healing by alleviating oxidative damage at the wound site. R7B3G3 treatment promoted the fastest wound healing. • The survival rate and quality of grafted seedlings were optimal when the cutting was performed within 3 d. Rapid healing in high-density environments and rooting during the cutting stage are the main challenges in two-stage grafted tomato seedling cultivation. Previous studies have indicated that green light may have a growth-promoting effect on plants. To verify the role of green light, white light (W), red:blue light at a 7:3 ratio (R7B3), and R7B3 supplemented with 10%, 20%, or 30% green light (R7B3G1, R7B3G2, and R7B3G3, respectively) were used during the healing period of the two-stage grafting. The results showed that the grafted seedlings treated with R7B3G2 and R7B3G3 exhibited the highest survival rates. R7B3G3 treatment promoted the fastest healing, with better-developed isolation layers and healing tissues, higher antioxidant enzyme activities and carotenoid content, and lower superoxide anion content at the wound site. There was a higher survival rate of cuttings after 3 d of high-density healing treatment. Among them, R7B3G2...