18 F‐FAPI‐74 Positron Emission Tomography/Computed Tomography Noninvasively Monitoring the Fibrosis of Cardiac Allograft Vasculopathy

作者：Huiling Li, Yufei Wei, Zhuoqi Qin, Qiyao Cheng, Hong Yu, Huibin Ru, Ting Song, Li Li, Xinzhong Hao, Jianhua Jin, Yan Cheng, Zhifang Wu, Sijin Li · 发表于：Journal of the American Heart Association · 年份：2025 · DOI：10.1161/jaha.125.041169 · 被引用次数：1 · 研究领域：Peptidase Inhibition and Analysis、Cardiac Fibrosis and Remodeling、Heat shock proteins research

Background Cardiac allograft vasculopathy (CAV) is the primary cause of limited long‐term survival among heart transplant recipients. This study aimed to evaluate the feasibility of 18 F‐FAPI‐74 positron emission tomography/computed tomography (PET/CT) imaging for monitoring the fibrosis of CAV. Methods Rat models of CAV were established and 1 hour dynamic PET/CT imaging was performed in the 15th week postoperation. Serial static 18 F‐FAPI‐74 PET/CT imaging in the allograft and isograft groups were performed from week 3 to 17. Blocking experiments were performed on allograft rats to confirm the tracer’s targeting specificity. In vivo PET/CT imaging findings were further corroborated by ex vivo PET/CT imaging and biodistribution studies. Histological examination was conducted to validate the model’s effectiveness, and the correlation between FAP expression levels and imaging results was ascertained. Results Dynamic PET/CT imaging revealed peak graft heart (Ht) uptake in the allograft group at 60 minutes post 18 F‐FAPI‐74 injection. PET/CT imaging and biodistribution studies at all time points confirmed significantly higher radioactivity uptake in Ht of the allograft group, as compared with both their native hearts and Ht of the isograft group, and peaking at week 15. There was almost no radioactive accumulation of Ht in the allograft rats blocked by FAPI. Histological analysis confirmed significantly elevated FAP expression in Ht of the allograft group compared with the isogra...