PpSnRK1α-PpNAC6/PpNAC36 module mediates nitrogen-regulated biosynthesis of γ-decalactone in peach fruit

作者：Jiahui Liang, Xin Zheng, Xuelian Wu, Zhe Wang, Zixuan Li, Yuansong Xiao, Jian Guo, Qiuju Chen, Jingjing Luo, Huaifeng Gao, Yangyang Gao, Futian Peng · 发表于：Horticulture Research · 年份：2025 · DOI：10.1093/hr/uhaf256 · 被引用次数：1 · 研究领域：Plant nutrient uptake and metabolism、Microbial Metabolites in Food Biotechnology、Layered Double Hydroxides Synthesis and Applications

Abstract Flavor-related compounds, particularly γ-decalactone—the key contributor to the characteristic ‘peach-like’ aroma—serve as essential indicators of peach fruit quality and strongly influence consumer purchasing decisions. However, excessive application of N fertilizers has led to a significant decline in the flavor quality of peaches, posing a major obstacle to the sustainable development of the peach industry. Although this remains a critical challenge, the molecular mechanisms linking N to flavor compound biosynthesis are still not well characterized. In this study, we discovered that excessive N application reduced the biosynthesis of γ-decalactone in peach, based on multi-year field observations. Correlation analysis and expression profiling under N treatments revealed that two NAC (NAM-ATAF1/2-CUC2) transcription factors (TFs), PpNAC6 and PpNAC36, were involved in regulating γ-decalactone biosynthesis in response to N signaling. Genetic analyses indicated that PpNAC6 and PpNAC36 positively regulated the accumulation of γ-decalactone. Both yeast one-hybrid (Y1H) assays and dual-luciferase reporter assays consistently showed that PpNAC6 and PpNAC36 directly interact with the promoter regions of γ-decalactone biosynthesis-related genes (PpAAT2, PpAAT3, PpLOX1, PpLOX6, and PpFAD3) and significantly enhance their transcriptional activity. Furthermore, transgene verification demonstrated that the α subunit of peach SNF-related Kinase 1 (PpSnRK1α) suppresses γ-decalacto...