Thermostable Xylanase from Chenopodium album : Thermodynamic and Kinetic Characterization with Sustainable Application in Agro-Industrial Biobleaching

作者：Khalil ur Rehman, Ehab A. Abdelrahman, Karma Albalawi, Mohammed Alissa, Ahmed A. Al‐Ghamdi, Suad A. Alghamdi, Alaa S. Alhegaili, Mohammed A. Alshehri, Mortaga M. Abou–Krisha, Abdulrahman G. Alhamzani · 发表于：Journal of Agricultural and Food Chemistry · 年份：2025 · DOI：10.1021/acs.jafc.5c05447 · 被引用次数：2 · 研究领域：Biofuel production and bioconversion、Enzyme Production and Characterization

at 70 °C) indicates increased molecular ordering during thermal denaturation, particularly in the presence of sorbitol, which acted as a stabilizing polyol, enhancing the conformational rigidity of the enzyme. The enzyme's biotechnological relevance was further validated through kraft pulp biobleaching trials. Treatment with 100 U/g of xylanase led to a 32% reduction in the Kappa number, an increase in brightness from 38.5% to 51.2% ISO, and minimal losses in viscosity (-4.8%) and yield (95.6%), confirming selective delignification without compromising cellulose integrity. These findings underscore the enzyme's promise in reducing chemical load during pulp biobleaching and efficiently producing xylo-oligosaccharides, which possess recognized prebiotic and nutraceutical value.