High-throughput sequencing unveils microbial succession patterns in restored Hulun Buir Sandy Land, northern China

作者：Tiantian Peng, Haojing Hao, Xiao Guan, Junsheng Li, Zhaoyan Diao, He Bu, Qiang Wo, Ni Song · 发表于：Journal of Arid Land · 年份：2025 · DOI：10.1007/s40333-025-0026-6 · 被引用次数：1 · 研究领域：Microbial Community Ecology and Physiology、Genomics and Phylogenetic Studies、Geology and Paleoclimatology Research

Abstract In recent years, intensive human activities have increased the intensity of desertification, driving continual desertification process of peripheral meadows. To investigate the effects of restoration on soil microbial communities, we analyzed vegetation–soil relationships in the Hulun Buir Sandy Land, northern China. Through the use of high-throughput sequencing, we examined the structure and diversity in the bacterial and fungal communities within the 0–20 cm soil layer after 9–15 a of restoration. Different slope positions were analyzed and spatial heterogeneity was assessed. The results showed progressive improvements in soil properties and vegetation with the increase of restoration duration, and the following order was as follows: bottom slope>middle slope>crest slope. During the restoration in the Hulun Buir Sandy Land, the bacterial communities were dominated by Proteobacteria, Actinobacteria, and Acidobacteria, whereas the fungal communities were dominated by Ascomycota and Basidiomycota. Eutrophic bacterial abundance increased with the restoration duration, whereas oligotrophic bacterial and fungal abundance levels decreased. The soil bacterial abundance significantly increased with the increasing restoration duration, whereas the fungal diversity decreased after 11 a of restoration, except that at the crest slope. Redundancy analysis showed that pH, soil moisture content, total nitrogen, and vegetation-related factors affected the bacterial community ...