The impact of altered intestinal microbiota on intestinal immune function after acute exhaustive exercise in mice

作者：Guang Yang, Lina Gao, Yuqian Liu, Xiaoyang Xu, Wu Yang · 发表于：BMC Sports Science Medicine and Rehabilitation · 年份：2025 · DOI：10.1186/s13102-025-01334-9 · 被引用次数：3 · 研究领域：Gut microbiota and health、Exercise and Physiological Responses、Clostridium difficile and Clostridium perfringens research

PURPOSE: Long-term training or intense exercise alters gut microbiota. This study aimed to determine the effects of microbiota on colonic permeability and immune function in mice subjected to acute exhaustive exercise. METHODS: C57BL/6 mice were randomly divided into the blank control (C), no exercise experience (NE), under a training protocol (E), phosphate-buffered saline (PBS) transplantation (PT), and fecal microbiota transplantation (FMT) groups. The E group underwent 14 weeks of moderate intensity training. At the end of the 14th week, fecal suspensions were prepared from mice in Group E and transplanted into Group FMT via enema, while Group PT received PBS enemas twice daily for 7 days. Prior to transplantation, both Groups PT and FMT were gavaged with antibiotics for 7 days, followed by 3 days of polyethylene glycol bowel cleansing. The C group was euthanized after a rest period, and the other groups were euthanized after acute exhaustive exercise. Colonic zonulin, occludin, ZO-1, CD14, TLR-4, MD-2, and TNF-α protein levels were detected via western blot, and enzyme-linked immunosorbent assays were used to detect serum LPS, IL-6, and colonic sIgA. RESULTS: Colonic zonulin protein expression was significantly higher (P < 0.01) and occludin and ZO-1 expression levels were significantly lower in the NE, PT, and FMT groups compared with the C group (P < 0.01). ZO-1 was significantly higher in the FMT group compared with the PT group (P < 0.05). Colonic MD-2, TLR-4, and CD...