Dysregulated serum lipid profiles in neovascular age-related macular degeneration revealed by UPLC‒MS/MS lipidomics

作者：Yuan Qu, Yujuan Liu, Junwei Fang, Chong Chen, Lu Cheng, Xiaoyin Xu, Jin Jing, Xia Chen, Tian Niu, Hanying Wang, Xindan Xing, Xin Shi, Yinchen Shen, Kun Liu · 发表于：Lipids in Health and Disease · 年份：2025 · DOI：10.1186/s12944-025-02735-y · 被引用次数：3 · 研究领域：Retinal Diseases and Treatments、Metabolomics and Mass Spectrometry Studies、Retinal Imaging and Analysis

PURPOSE: Neovascular age-related macular degeneration (nAMD) is a major aetiology of vision loss characterized by lipid metabolism dysregulation. This study investigated the alterations in lipid profiles associated with wet AMD (wAMD) and its subtype, polypoidal choroidal vasculopathy (PCV). METHODS: A cross-sectional lipidomic analysis was derived from a prospective longitudinal study in China (2017-2019). Serum samples from 195 wAMD patients, 130 PCV patients and 119 controls were analysed using ultra-performance liquid chromatography‒tandem mass spectrometry (UPLC‒MS/MS), focusing on acylcarnitines, glycerophospholipids and sphingolipids. Training and test sets were randomly generated after sample size matching. Orthogonal partial least squares-discriminant (OPLS-DA), univariate and multivariate analyses were performed. The selection criteria for differential lipids were a variable importance in projection (VIP) > 1.0, a fold change (FC) > 1.2 or < 0.83, and a false discovery rate (FDR) < 0.05. Key lipids were refined using the Boruta algorithm and evaluated through Firth regression and receiver operating characteristic (ROC) analysis. Spearman's rank correlation analysis was used to assess the relationships between the differential lipids and ocular indicators, including central retinal thickness (CRT), central retinal volume (CRV) and best-corrected visual acuity (BCVA). RESULTS: No significant Lipid profile differences were detected between wAMD and PCV. Compared with t...