High-Performance Silicon Nanowire Array Biosensor for Combined Detection of Colorectal Cancer Biomarkers

作者：Jiaye Zeng, Mingbin Liu, Xin Chen, Jintao Yi, Wenhe Liu, Xinjian Qu, Chaoran Liu, Serestina Viriri, Guangguang Yang, Xun Yang, Weichao Yang · 发表于：Micromachines · 年份：2025 · DOI：10.3390/mi16101089 · 被引用次数：4 · 研究领域：Advanced biosensing and bioanalysis techniques、Nanowire Synthesis and Applications、Biosensors and Analytical Detection

This study presents a high-performance silicon nanowire (SiNW) array biosensor for the combined detection of two key colorectal cancer (CRC) biomarkers: circulating tumor DNA (ctDNA) and carcinoembryonic antigen (CEA). The device was fabricated using conventional micromachining techniques, enabling the integration of dual SiNW arrays on a single chip with precise control over structure and surface functionalization. Specific probe DNA and anti-CEA antibodies were immobilized on distinct array regions to facilitate targeted binding. The biosensor demonstrated exceptional performance, achieving an ultralow detection limit of 10 aM for ctDNA with a linear range from 0.1 fM to 10 pM, and a sensitivity of 1 fg/mL for CEA. It exhibited high selectivity against interfering substances, including single-base mismatched DNA and non-specific proteins, and maintained robust performance in human serum samples. The platform offers a scalable, label-free, and real-time detection solution with significant potential for application in early CRC screening and personalized medicine.