Isolation of a Novel Streptomyces Species from the Tuha Basin and Genomic Insights into Its Environmental Adaptability

作者：Xiaomin Niu, Yujie Wu, Xue Yu, Shiyu Wu, Gaosen Zhang, Guangxiu Liu, Tuo Chen, Wei Zhang · 发表于：Microorganisms · 年份：2025 · DOI：10.3390/microorganisms13102238 · 被引用次数：2 · 研究领域：Microbial Natural Products and Biosynthesis、Genomics and Phylogenetic Studies、Microbial Community Ecology and Physiology

Mining novel Streptomyces species from extreme environments provides a valuable strategy for the discovery of new antibiotics. Here, we report a strain of Streptomyces sp. HMX87T, which exhibits antimicrobial activity and was isolated from desert soil collected in the Tuha Basin, China. Molecular taxonomic analysis revealed that the 16S rRNA gene sequence of strain HMX87T shares the highest similarity with those of Streptomyces bellus CGMCC 4.1376T (98.5%) and Streptomyces coerulescens DSM 40146T (98.43%). In phylogenetic trees, it formed a distinct branch. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HMX87T and the above two type strains were below the thresholds of 95% and 70%, respectively, confirming that strain HMX87T represents a novel species within the genus Streptomyces, for which the name Streptomyces hamibioticus sp. nov. is proposed. Physiologically, the strain HMX87T grew at temperatures ranging from 25 to 37 °C, tolerated pH values from 5 to 12, and survived in NaCl concentrations of 0% to 8% (w/v). Chemotaxonomic characterization indicated the presence of LL-diaminopimelic acid (LL-DAP) in the cell wall, ribose and galactose as whole-cell hydrolysate sugars, MK-9(H8) (66.3%) as the predominant menaquinone, and iso-C16:0 (25.94%) and anteiso-C15:0 (16.98%) as the major fatty acids characteristics that clearly distinguish it from its closest relatives. Whole-genome sequencing of strain HMX87T revealed an abu...