An Interpretable SERS–AI Platform for Rapid and Quantitative Diagnosis of Polymicrobial UTIs: Powered by Positively Charged Plasmonic Nanoparticles and Attention‐Based Deep Learning

作者：Zhonghua Shen, Linguo Xie, Y. Hou, Junjie Liang, Yuchi Jia, Haipeng Zhang, Zhenli Sun, Jingjing Du, Zhaofan He, Chunyu Liu, Wenjing Liu · 发表于：Advanced Science · 年份：2025 · DOI：10.1002/advs.202513502 · 被引用次数：13 · 研究领域：Bacterial Identification and Susceptibility Testing、Biosensors and Analytical Detection、Spectroscopy Techniques in Biomedical and Chemical Research

Abstract Polymicrobial urinary tract infections (UTIs) present diagnostic challenges due to overlapping symptoms and limitations of conventional methods. Although SERS and AI have shown potential for microbial diagnostics, existing approaches lack reproducibility, quantification capability, and interpretability—especially in complex clinical samples. Here, a label‐free, interpretable SERS‐AI platform for rapid identification and quantification of mixed urinary tract pathogens is proposed. A plasmonic substrate is engineered by combining Au@Ag core–shell nanoparticles with a positively charged bPEI surface, enabling electrostatic bacterial capture and stable SERS signal generation across diverse microbial mixtures. A convolutional neural network (CNN) enhanced with a convolutional block attention module (CBAM) to enable both accurate classification (95.8%, AUC = 0.9774) and reliable bacterial proportion prediction (R 2 = 0.9112), surpassing traditional models, is developed. Importantly, the attention mechanism offers mechanistic interpretability, highlighting biologically relevant spectral features related to nucleic acids, proteins, and virulence factors. Validation with clinical urine samples demonstrates strong predictive performance (accuracy = 86.9%, R 2 = 0.8626), supporting real‐world applicability. Overall, this work not only delivers a high‐throughput and explainable framework for polymicrobial diagnostics, but also contributes to the mechanistic understanding of Rama...