Stenotrophomonas muris —first discovered as a potential human pathogen with strong virulence and antibiotic resistance, associated with bloodstream infections

作者：Jiaying Liu, Xu Dong, Yanghui Xiang, Yi Li, Yuyun Yu, Tiantian Wu, Xin Yuan, Dan Cao, Hanyin Zhang, Lixia Zhu, Ying Zhang · 发表于：Microbiology Spectrum · 年份：2025 · DOI：10.1128/spectrum.02770-24 · 被引用次数：5 · 研究领域：Infections and bacterial resistance、Blood disorders and treatments、Mycobacterium research and diagnosis

ABSTRACT For the first time, Stenotrophomonas muris , whose pathogenic potential for humans has never been reported previously, has been identified to be associated with human infections. In this work, the phenotype of S. muris virulence, the potential genes that encode higher virulence of S. muris , and host responses to S. muris infection were investigated for the first time. It was found that S9 ( S. muris no. 9, isolated from the patient’s bloodstream infection) was more virulent than both S8 ( S. muris no. 8, isolated from the patient’s sputum) and S1 ( Stenotrophomonas maltophilia type strain ATCC13637). Candidate genes that may encode higher virulence of S9 were identified, including virB6 , dcm , hlyD , and 14 other genes involved in porphyrin metabolism, pyrimidine metabolism, DNA methylation, two-component system, and biofilm formation. Transcriptome analysis of infected host cells (THP-1 cells) showed that 13 candidate genes involved in host response to hypoxia ( HILPDA , FOXH1 , ANGPTL4 , SLC2A1 , HK2 , ADM , CXCR4 , BNIP3 , PLAT , PLOD2 , STC2 , STC1 , and AK4 ) were preferentially upregulated by the more virulent strain S9 over the less virulent S8. Two downregulated genes ( SLC26A11 and SLC8A1 ) involved in monoatomic ion transport and the calcium signaling pathway may also need special attention, as they may be involved in pathogenesis. Antibiotic susceptibility testing indicated that strains S8 and S9 demonstrated high resistance to colistin and polymyxin B, ...