Genome sequencing and experimental validation reveal the biocontrol activity of endophytic Bacillus velezensis XY3 against Colletotrichum fructicola

作者：Yimei Zhang, Ziyang Duan, Yi Lv, Zhenkun Li, Wanying Zhang, Lingyun Zheng, Ya-Feng Dai, Guang Yang, An‐Dong Gong · 发表于：Biological Control · 年份：2025 · DOI：10.1016/j.biocontrol.2025.105892 · 被引用次数：3 · 研究领域：Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity、Mycotoxins in Agriculture and Food

• XY3 inhibits conidial germination and hyphae growth of C. fructicola . • The EC 50 value of crude lipopeptides derived from XY3 was 21.33 μg/mL. • IturinA, fengycinA and surfactin of XY3 were examined by LC-MS/MS analysis. • IturinA and fengycinA showed antifungal activity against C. fructicola . Tea anthracnose, caused by Colletotrichum spp., is a prevalent foliar disease in the primary tea-planting regions of China. The conventional chemical control of tea anthracnose results in excessive fungicide residues, which constrain the export of tea from our country. Therefore, it is imperative to develop safe and pollution-free biological control methods. Strain XY3, isolated from the healthy leaves of ‘Xinyang 10’ cultivar and identified as Bacillus velezensis through 16S rRNA gene analysis, demonstrated significant inhibition of conidial germination, hyphal growth, and the pathogenicity of Colletotrichum fructicola . Propidium iodide and Hoechst staining assays indicated that the membrane permeability of the mycelium was compromised when cultured with the fermentation broth of XY3. Crude lipopeptides were extracted from fermentation broth showing an EC 50 value of 21.33 µg mL −1 , and the antimicrobial compounds including iturinA, fengycinA, surfactin, and their homologs were detected via LC-MS/MS. Plate confrontation assay verified that iturin and fengycin purified compounds exhibited notable inhibitory activities. Additionally, the whole genome of XY3 was sequenced with 46.5...