Mechanistic insight into the frequency-dependent ultrasound-assisted extraction of Rosa laevigata Polysaccharides: Structure, antioxidant activity, and process optimization

作者：Yuyuan Duan, Shuting Wang, Xiaorong Zhang, Huimei Zhang, Huizhu Wang, Shuai Chen · 发表于：Ultrasonics Sonochemistry · 年份：2025 · DOI：10.1016/j.ultsonch.2025.107577 · 被引用次数：10 · 研究领域：Polysaccharides and Plant Cell Walls、Microbial Metabolites in Food Biotechnology、Polysaccharides Composition and Applications

Rosa laevigata Michx. polysaccharide (RLMP) possess considerable bioactive potential; however, the frequency-dependent mechanisms underlying their ultrasound-assisted extraction (UAE) remain insufficiently understood. This study systematically investigated the influence of ultrasonic frequency on the extraction efficiency, structural characteristics, and antioxidant activity of RLMP, with the goal of optimizing the UAE process. Single-frequency ultrasound (SFU) at 20, 40, and 53 kHz and dual-frequency ultrasound (DFU) at 20/40 and 20/53 kHz were compared. Results demonstrated that 40 kHz SFU achieved the highest extraction yield, accompanied by higher glucose content, favorable molecular weight, and reduced protein contamination. In contrast, DFU treatments resulted in lower yields and elevated protein levels, indicating destructive wave interference rather than synergistic effects. Structural analyses further revealed that ultrasonic frequency significantly influenced monosaccharide composition, molecular weight distribution, and surface morphology, while the primary chemical structure remained unchanged. RLMP extracted at 40 kHz exhibited superior antioxidant activity, with more effective scavenging of diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and superoxide anion radicals. Cellular antioxidant assays further confirmed its protective effects, showing a 42.83 % reduction in intracellular reactive oxygen species and enhanced superoxide...