Phylogenomic Identification of a Highly Conserved Copper-Binding RiPP Biosynthetic Gene Cluster in Marine Microbulbifer Bacteria

作者：Yifan Tang, Weimao Zhong, Longping Fu, Emmanuel Asante, Anastasiia Kostenko, F. N. U. Vidya, Paige Mandelare-Ruiz, Tamilore T. Adeogun, Gabriel P. Anderson, Benjamin E. Edmonds, Fang Ou, Michelle Han, Alan B. Hollingsworth, Amna R. Ingham, C. Kirby, Alice Landrum, C. Mack, Nikki S. Nobari, Emma J. Oswald, Cecilia L. Polevoy, Yasmin Sharifian, T. So, Joelee R. Stokes, Reniya S. Thompson, Rishabh Vuthamaraju, Elaine Wang, William H. Yang, Alison E. Onstine, Valerie J. Paul, Ronghu Wu, Allegra T. Aron, Vinayak Agarwal · 发表于：ACS Chemical Biology · 年份：2025 · DOI：10.1021/acschembio.5c00507 · 被引用次数：14 · 研究领域：Genomics and Phylogenetic Studies、Microbial Natural Products and Biosynthesis、Enzyme Production and Characterization

High Resolution Image Download MS PowerPoint Slide Conserved biosynthetic gene clusters (BGCs) are often tied to the production of natural products that perform critical functions in an organism’s physiology and ecological interactions. Here, by phylogenetic analysis across the bacterial genus, we report the obligate conservation of a BGC in genomes of cosmopolitan marine Microbulbifer bacteria. This genus is a common member of marine microbiomes, and this BGC was conserved in Microbulbifer genomes regardless of phylogenetic or geographical dispersal. The post-translationally modified peptidic product encoded by this BGC─which was accessed via heterologous production and its structure elucidated using a combination of mass spectrometry and NMR spectroscopy─was found to be a copper chelator. Similar BGCs were then found in genomes of other marine bacterial genera coinhabiting the microbiomes of sponges and corals. The phylogenomic workflows described herein were implemented in a pedagogic setting at the Georgia Institute of Technology to provide hands-on instruction to undergraduate students in bacterial phylogeny, genome mining, and natural product chemistry.