Towards a comprehensive barcoding and phylogenomic reference for the European arctic–alpine flora

作者：Sébastien Lavergne, Charles Pouchon, Cristina Roquet, Adriana Alberti, Martí Boleda, Christophe Perrier, Julien Renaud, Frédéric Boyer, Bruno Bzeznik, Maxime Rome, Rolland Douzet, Youri Lammers, Céline Orvain, Frédérick Gavory, Karine Labadie, S. Aubert, PhyloNorway Consortium, PhyloAlps Consortium, Patrick Wincker, Jean-Gabriel Valay, Niklaus E. Zimmermann, Wilfried Thuiller, France Denœud, Inger Greve Alsos, Éric Coissac · 发表于：Botany Letters · 年份：2025 · DOI：10.1080/23818107.2025.2549565 · 被引用次数：5 · 研究领域：Botany and Plant Ecology Studies、Bryophyte Studies and Records、Plant Taxonomy and Phylogenetics

We present a decade-long research initiative of biodiversity genomics, which aimed at sequencing all vascular plant species occurring in the biodiversity hotspot of the European Alps, along with related lineages occurring in other mountain ranges and the arctic region. We detail the sampling and sequencing strategies, an online database of genome skimming datasets, and bioinformatic procedures to retrieve sequence data. Based on low coverage shotgun sequencing of 6,105 species, we generated a dataset of 81 orthologous chloroplast loci, including all standard barcodes. Using maximum likelihood, we inferred a species-resolved phylogeny which covers 96% of the flora occurring above treeline in the Alps and 92% of the Arctic Flora, along with many additional non-alpine species to improve species sampling and allow fossil dating. The data released here provide the baseline of a unique, ever-growing genomic resource for various applications such as phylogenomics, systematics, conservation and metabarcoding of the arctic–alpine realm.