Low-phosphorus stress induces GmSTOP1-3-mediated organic acid exudation to recruit phosphate-solubilizing bacteria for organic phosphorus mineralization in soybean rhizosphere

作者：Qianqian Chen, Xing Lu, Guoxuan Liu, Tianqi Wang, Huiying Zhou, Jihui Tian, Jihui Tian, Qing Yao, Jinming He, Jiang Tian, Jiang Tian, Cuiyue Liang · 发表于：Journal of Integrative Agriculture · 年份：2025 · DOI：10.1016/j.jia.2025.08.015 · 被引用次数：6 · 研究领域：Plant nutrient uptake and metabolism、Legume Nitrogen Fixing Symbiosis、Soil Carbon and Nitrogen Dynamics

• Expression of GmSTOP1-3 is up-regulated by phosphate starvation in soybean roots. • GmSTOP1-3 regulates root organic acid exudation and facilitates soil P utilization. • GmSTOP1-3 -mediated organic acid exudation recruits phosphate-solubilizing bacteria. Root exudates serve a vital function in recruiting beneficial phosphate-solubilizing bacteria (PSB), thereby enhancing plant adaptation to phosphorus (P) deficiency. The C2H2-type zinc finger transcription factor STOP1 (sensitive to proton rhizotoxicity1) regulates root organic acid (OA) exudation in plants. However, the impact of STOP1-regulated root OA exudation on rhizosphere microbial composition remains unexplored. This study revealed enhanced vegetation properties of soybean with higher P content in P-rich soils, while rhizosphere organic acid concentrations were elevated in P-poor soils. The soybean genotype YC03-3 in P-deficient soils specifically recruited three PSB in acid soils: Gammaproteobacteria_Incertae_Sedis, KF_JG30_C25, and Solirubrobacterales. These PSB abundances correlated positively with rhizosphere oxalate and citrate concentrations. Under P-sufficient conditions, GmSTOP1-3 overexpression in soybean plants increased oxalate and citrate exudation compared to wild-type (WT) plants, leading to preferential colonization by the same three PSB species naturally present in P-deficient WT rhizosphere. The population dynamics of these PSB demonstrated strong positive correlations with the abundance of key gene...