Ozone water enema activates SIRT1-Nrf2/HO-1 pathway to ameliorate gut dysbiosis in mice receiving COVID-19 patient-derived faecal microbiota

作者：Zehua Su, Jiaqi Lin, Xuejiao Zeng, Xin Li, Qianhao Hou, Qing Wang, Chunzheng Liu, Jiawen Qin, Y Li, Jinyuan Zhang, Xiangrui Wang, Shuwen Qian, Lijun Liao · 发表于：Journal of Medical Microbiology · 年份：2025 · DOI：10.1099/jmm.0.002038 · 被引用次数：3 · 研究领域：Medical and Biological Ozone Research、Hydrogen's biological and therapeutic effects、Climate Change and Health Impacts

Introduction. This study centres on how coronavirus disease 2019 (COVID-19) disrupts the intestinal microbiota and amplifies systemic inflammation and evaluates ozone water enemas as a strategy to restore gut microbial balance and activate the SIRT1 (silent information regulator of transcription 1)-Nrf2 (nuclear factor erythroid 2-related factor 2)/HO-1 (heme oxygenase-1) pathway for alleviating post-viral sequelae. Our findings demonstrate that ozone water intervention markedly improves the intestinal microenvironment in mice receiving COVID-19 patient-derived microbiota and attenuates systemic inflammation, offering a viable adjunctive approach for COVID-19 management. Hypothesis. Despite significant progress in reducing the incidence of COVID-19, its long-term consequences, including hepatic dysfunction, pulmonary injury and gut microbiota dysbiosis, remain challenging. While ozonated water enema therapy has shown efficacy in alleviating inflammation and neutralizing oxidative stress, the precise mechanisms by which ozonated water attenuates COVID-19 progression are not fully understood. We hypothesized that ozonated water enemas could enrich gut microbiota composition in COVID-19 patients, thereby optimizing the gut environment following faecal transplantation in a murine model. Aim. The overarching aim of this investigation was to ascertain whether ozonated water enemas could exert a salutogenic effect on the gut microbiota in a mouse model, as well as on the holistic gu...