Ultrasmall Self-Cascade Bimetallic Au 2 Pd 3 Nanozymes Encapsulated Hydrogel Microneedles for Photothermal-Augmented Nanocatalytic Therapy of Diabetic Wound Infections

作者：Weiwei Chen, Fan Yang, Xi Chen, Cheng Jin, Ninghong Yin, Mengya Sun, Yawen Zheng, Ke Yang, Zefeng Wang, Dinggeng He, Li Wang, Luo Hai · 发表于：ACS Applied Materials & Interfaces · 年份：2025 · DOI：10.1021/acsami.5c14298 · 被引用次数：8 · 研究领域：Advancements in Transdermal Drug Delivery、Nanoplatforms for cancer theranostics、Wound Healing and Treatments

An ultrasmall bimetallic Au 2 Pd 3 nanozyme with self-cascade enzyme-like activities and robust photothermal heating properties is synthesized and encapsulated into a poly(vinyl alcohol)/hyaluronic acid (PVA/HA) matrix via a repeated freezing and thawing process to yield a hydrogel microneedle patch (Au 2 Pd 3 @PH). This microneedle displays a good tissue-piercing capability and thermal-responsive melting behavior. Upon exposure to an 808 nm near-infrared laser, Au 2 Pd 3 @PH dissolves and releases Au 2 Pd 3, which shows glucose oxidase (GOx)-like catalytic activity for the conversion of endogenous glucose into gluconic acid and hydrogen peroxide (H 2 O 2 ), thereby reducing the glucose level and local pH at the wound site. The reduced pH induces the peroxidase-like activity of Au 2 Pd 3 to further oxidize H 2 O 2 into the hydroxyl radical ( • OH). In vitro experiments reveal that Au 2 Pd 3 @PH exhibits antibacterial rates exceeding 99% against both Staphylococcus aureus and Salmonella typhimurium, showing a photothermal/chemodynamic dual-mode synergistic antibacterial effect. Further in vivo assays demonstrate that the Au 2 Pd 3 @PH treatment group achieves near-complete healing of infected diabetic wounds within 7 days, indicating an enhanced wound healing process. The treatment with Au 2 Pd 3 @PH effectively modulates inflammatory factor expression, including marked downregulation of TNF-α and IL-6, while simultaneously promoting collagen deposition and angiogenesis, with ...