Combined BSA-seq and RNA-seq approaches reveal candidate genes associated with seed weight in Brassica napus

作者：Xinxin Geng, Fengling Yang, Wenhua Tang, Ying Wang, Fu Shan, Zichen Yu, Wanli Cheng, Liang Chen, Xiaomeng Xue · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2025.1678464 · 被引用次数：4 · 研究领域：Plant Molecular Biology Research、Plant Gene Expression Analysis、Plant-Microbe Interactions and Immunity

Brassica napus , a globally significant oilseed crop of the Brassicaceae family, serves as a major source of vegetable oil and biofuel. Seed size/weight is a crucial agronomic trait that directly determines crop yield. However, the genetic mechanisms underlying seed weight in B . napus has not been fully understood. In this study, R140 with extremely low thousand-seed weight (2.6g) was crossed with Zhongshuang 11 (4.9g) to construct an F 2 population. Five major genomic regions on chromosomes A06 (43.88-44.63Mb), A08 (27.63-27.68Mb), A09 (55.32-55.46Mb, 57.33-57.58Mb), and C07 (29.40-29.60Mb) were identified as candidate loci of seed weight via BSA-seq approach. A total of 204 genes were annotated within the candidate regions, including 103 non-synonymous mutant genes and 26 frameshift mutant genes identified between parental lines. Among them, 21 DEGs were screened through RNA-seq analysis of the developing seeds in both parents. However, only 8 genes exhibited mutations in their coding or upstream sequences, which were characterized as the candidate genes associated with the small seed phenotype of R140. An auxin response factor18 coding gene ( BnARF18 ) exhibited significantly differential expression between parents. Analysis of the promoter element variations revealed that the MYC-motif, implicated in gene expression regulation, and the WUN-motif, associated with cell differentiation and proliferation control, likely serve as key regulatory motifs responsible for the diff...