In vitro antibacterial and In vivo osteogenesis of 3D-printed magnesium peroxide–doped calcium phosphate silicate scaffolds for revision total knee arthroplasty

作者：Lisha Meng, Hao Li, Xujia Hao, Tao Wu, Jingqiu Zhou, Yadong Chen, Qiang Zheng, Xiuhong Cao, Juan Wang, Xinwei Liu, Tongmeng Jiang, Tianxing Gong, Wei Yuan · 发表于：Materials & Design · 年份：2025 · DOI：10.1016/j.matdes.2025.114731 · 被引用次数：2 · 研究领域：Bone Tissue Engineering Materials、Total Knee Arthroplasty Outcomes、Orthopaedic implants and arthroplasty

• Effective antibacterial efficacy against MRSA, achieved through sustained magnesium and hydrogen peroxide release. • Biocompatible and stable scaffold with optimized porosity and mechanics designed for effective bone defect repair. • Enhanced bone regeneration and osteointegration in vivo confirmed by micro-CT, histology, and immunohistochemical markers. Revision total knee arthroplasty (RTKA) often encounters tibial bone defects and high infection risk, especially from methicillin-resistant Staphylococcus aureus (MRSA). Current strategies rely on bone grafts with antibiotics, but prolonged use promotes resistance. Here, we developed a 3D-printed magnesium peroxide (MgO 2 )–doped calcium phosphate silicate (CSP) scaffold to address both structural and antibacterial demands. The MgO 2 –CSP scaffold exhibited cancellous bone-like strength (∼7.95 MPa) and an interconnected macroporous structure conducive to cell migration and healing. In vitro , the 14 wt% MgO 2 scaffold (B14M) inhibited 80.4 % of Gram-negative bacteria and 74.6 % of MRSA via Mg 2+ and H 2 O 2 release, while both B0M (no MgO 2 ) and B14M promoted BMSC proliferation and osteogenic differentiation. In vivo , the B14M scaffold markedly enhanced bone regeneration in rat tibial defects, achieving a BV/TV of ∼73.09 % versus ∼29.84 % for B0M at 8 weeks. These findings highlight MgO 2 –CSP scaffolds as a promising strategy to promote osteogenesis while combating MRSA-associated infections in RTKA.